Treatment of experimental animals with dgalactosamine galn lipopolysaccharide lps causes lethal liver injury that is characterized by apoptosis of the hepatocyte. Hepatocyte apoptosis in dgalnlpsinduced liver injury. The aim of the present wok the hepatoprotective activity as well as the antioxidant potential of the ethyl acetate stem extract of m. Hepatoprotective activity of lepidium sativum seeds against dgalactosamine lipopolysaccharide induced hepatotoxicity in animal model. Molecular mechanisms of dgalactosaminelipopolysaccharide. The appearance of dgalactosamineinduced hepatitis and generalized edema in. In addition, it was reported that jnk1, but not jnk2, promoted liver cancer progression in a mouse model of chemicalinduced liver carcinogenesis.
Hepatoprotective activity of lepidium sativum seeds against dgalactosaminelipopolysaccharide induced hepatotoxicity in animal model. The present study was conducted to investigate effect of pentoxifylline ptx on acute liver injury caused by galactosamine dgal in rats and the underlying. Against dgalactosamine induced hepatotoxicity in mice. Treatment with boswellia ovalifoliolata root extracts has protected liver from induced hepatotoxicity.
The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharideinduced hepatotoxicity of dgalactosamine sensitized rats. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Nacetyldgalactosamine galnac, an aminosugar, is a component of many olinked and nlinked glycan structures. The present study was designed to investigate the effect of carvacrol on dgalactosamine dgalninduced hepatotoxicity in rats. Frontiers asiatic acid exhibits antiinflammatory and. In colectomized rats, galn produced little hepatotoxicity, while it induced severe liver damage by simultaneous administration of. Acetylbergenin was administered orally once daily for 7 days and then galn. We aim to evaluate the protective effects and definite mechanism by which orally administered b. Sedum sarmentosum is traditionally used to treat various inflammatory diseases in china. Protective effect of wild ginseng cambial meristematic cells on dgalactosamineinduced hepatotoxicity in rats.
Manual of histology, staining methods of armed forces, institute of pathology. Dgalactosamine is an amino sugar derivative of dgalactose item no. Pdf hepatoprotective effects of punica granatum fruit against d. Compared with biofluids, target tissues and organs more directly reflect the pathophysiological state of a disease process. This was demonstrated by reducing the elevated levels of biochemical markers and additional histopathological observations have shown that there is an improvement in the structural design of liver due to induced hepatotoxicity.
As udpgalnac, galnac is the intial olinked sugar to many serine and threonine residues in protein glycosylations. Lps is a toxic component of the cell walls of gramnegative bacteria and is widely present in the digestive tracts of humans and animals 3. The present study was undertaken to determine whether dgalactosamine dgaln induces ali via the mitochondrial apoptosis and proinflammatory cytokinesignaling pathways, and possible mechanisms by which green tea gt extract modulates the apoptotic and proinflammatory signaling. Experimental and molecular patholocy 29, 8291 1978 dgalactosamine hepatotoxicity v.
Hepatoprotective activity of lepidium sativum seeds against d. However, overactivation thereof may lead to hepatocellular damage. In this study, danhong injection dhi was investigated to evaluate the preventive and protective effect on ahf induced by lipopolysaccharide lps and dgalactosamine galn in mice. It is a nacetyldhexosamine and a nacetylgalactosamine. Glycine and uridine prevent dgalactosamine hepatotoxicity in the rat. Effects of acetylbergenin against d galactosamineinduced. The present study was aimed to evaluate the hepatoprotective activity of ethanolic extract of roots of tetracera akara root on dgalactosamine induced hepatotoxicity in wistar rats. Antihyperlipidemic effect of carvacrol on dgalactosamine. Chronic liver disease such as hepatitis is a major health problem worldwide, especially in developing countries. Apoptosis plays a role in the normal development of liver. Glycine and uridine prevent dgalactosamine hepatotoxicity.
Hepatoprotective effects of punica granatum fruit against dgalactosamineinduced hepatotoxicity in rats. Ambrex was orally administered for a period of 7 days at dose levels of 250 and 500 mgkg b. Dgalnlps treatment was able to induce hepatotoxicity and significantly increase all markers of liver damage and lipid peroxidation. Evaluation of druginduced tissue injury by measuring. Protective role of catechin on dgalactosamine induced. Protective effect of aa treatment on lipopolysaccharide lps dgalactosamine galninduced fulminant hepatic failure. Pdf dgalactosamine is a hepatotoxic agent, which induces diffuse injury of. Effects of cultured acer tegmentosum cell extract against. Hepatoprotective effects of il22 on fulminant hepatic failure induced by dgalactosamine and lipopolysaccharide in mice. It has a role as a human metabolite, an escherichia coli metabolite and a mouse metabolite. We analyzed the molecular mechanism of galnlpsinduced apoptosis of hepatocytes and examined the therapeutic effects of etoposide on galn lpsinduced lethal liver injury.
Hepatotoxicity of dgalactosamine in the isolated perfused rat liver. Hepatotoxicity was induced in wistar rats by intraperitoneal injection of dgaln 400 mgkg in saline in wistar rats. In this study, a dgalactosamine galn lipopolysaccharide lpsinduced mouse model was constructed to investigate metabonomics of liver tissue and directly characterize metabolic changes in acute liver failure alf. Dgalactosamine is hepatotoxic and is used, alone or in combination with lps, as a model of liver failure in rodents. Frontiers pretreatment with bacillus cereus preserves.
In the absence of galactosamine, there was no loss of viability over this same period. Ameliorative effect of ethanolic extract of roots of. The aim of this study was to assess dgalactosamine dgalnlipopolysaccharide lpsinduced hepatocyte apoptotic changes in mice and clarify the mechanisms involved in this process. Ultraperformance liquid chromatography quadrupole timeofflight mass spectrometry uplcqtofms has been used for. Hepatoprotective effects of salidroside on fulminant. Liver transplant for viral hepatitis and fulminant hepatic failure. After 36 hours, only 1020% of the original cells were viable, as assessed by trypan blue exclusion. Hepatoprotective effects of purple potato extract against d. The seeds of lepidium sativum were identified by taxonomist in the college of pharmacy, king saud university, riyadh.
Metabonomic analysis of liver tissue from balbc mice with. A voucher specimen 32657 was deposited at the herbarium of the college of pharmacy, king saud university, riyadh, saudi arabia. Hepatoprotective activity of salidroside isolated from acer termentosum max on dgalactosamine induced hepatotoxicity in rats. Acute hepatic failure is clinically characterized by massive hepatocyte apoptosis and very high patient mortality. Jci insight mitochondrial dnaenriched microparticles. Drugs that induce hepatotoxic effects in mammals were injected into the silkworm. Objective of this study was to obtain a better understanding of the mechanism responsible for the dgalactosamine dgaln induced hepatotoxicity and to study the effect of catechin against dgaln induced hepatotoxicity. In fact, an experimental model of liver failure induced by d. It has protective effects against acute liver injury, but the exact mechanism of such effects remains unclear. Therefore, these results suggest that acetylbergenin has hepatoprotective effects against galninduced hepatotoxicity by inhibiting lipid peroxidation and. Full text pdf 1764k abstracts references20 citedby5 the role of endotoxin in liver injury induced by dgalactosamine galn was investigated in rats in vivo, or by using isolated rat hepatocytes in vitro. Role of free fatty acids in the pathogenesis of fatty liver i seymour m. Our previous studies suggest silkworms can be used as model animals instead of mammals in pharmacologic studies to develop novel therapeutic medicines.
Lipopolysaccharide lpsdgalactosamine dgaininduced liver injury has been widely used to study the mechanisms of human hepatitis and to examine hepatic protection of compounds. Lecithin 50 and 100 mgkg b wt was administered for 1 week by oral route. Extrahepatic factors, such as increased gut permeability and bacteria from the gut, have been shown to play a role ind. Hepatotoxic effect of dgalactosamine and protective role. Read hepatoprotective effects of salidroside on fulminant hepatic failure induced by d. This study examined the protective mechanism of wild ginseng cmcs against dgalactosamine galninduced liver injury.
Packaging 10 mg in glass bottle 5, 25 g in poly bottle. Galactosamineinduced cell death in primary cultures of. Thurman1,3 extrahepatic factors, such as increased gut permeability and bacteria from the gut, have been shown to play a role in. The present study investigated the protection by dietary spirulina platensis against dgalactosamine dgaln and acetaminophen apapinduced hepatitis in icr mice. Twentyone spraguedawley rats were equally assigned into three groups n 7 animals per group. Hepatoprotective activity of seeds against d galactosaminelipopolysaccharide induced hepatotoxicity in animal model. Lepidium sativum l seeds were procured from a local market in riyadh, saudi arabia.
Hepatoprotective activity of bacopa monniera on dgalactosamine induced hepatotoxicity in rats bacopa monniera. Twenty four wistar strain albino rats were used for the investigations. Dgalactosamine administration, which indicates a high extent of liver injury. Curcumin attenuates dgalactosaminelipopolysaccharide. Dgalactosamineinduced utp deficiency is reversed completely within 90 min after uridine administration. Multiple factors contribute to hepatic failure, including excessive use of alcohol, viral infection, liver transplant failure, the side effects of chemical drugs, and the metabolism of xenobiotics. This offers the possibility to inhibit utpdependent processes in vivo for periods corresponding to the time interval between galactosamine and uridine injection. Pretreatment with eeib and silymarin for 21days protected the rat livers from dgalactosamine induced hepatotoxicity. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt dgalactosamine on the. Liver diseases still represent a major health burden worldwide.
Pdf a mixture of mulberry and silk amino acids protected. Dgalactosaminelipopolysaccharideinduced hepatotoxicity. Alf is the most severe form of acute liver injury ali that typically manifests as hepatic dysfunction, coagulopathy, and encephalopathy. Selective uridine triphosphate deficiency induced by d. The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galn induced liver damage in rats, compared with that of bergenin reported previously. Ragland division of gastroenterology, university of tennessee center for the health sciences, and veterans administration hospital, memphis, tennessee 38163 received october 25, 1977, and in revised forma. Because bacterial endotoxin activates kupffer cells, the purpose of this study was to clarify the role of kupffer cells in the mechanism ofd. However, treatment with silymarin and liv52 significantly decrease the biochemical markers, as compared to dgalactosamine group. Protection by dietary spirulina platensis against d. Pdf hepatoprotective activity of lepidium sativum seeds. A mixture of mulberry and silk amino acids protected against d. Galn, a wellknown hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. Modification of the hepatotoxicity of dgalactosamine in.
Modification of the hepatotoxicity of dgalactosamine in the rat by cycloheximide. Increasing attention has been paid to spirulina for its potential clinical uses. Acute hepatic failure ahf, which leads to an extremely high mortality rate, has become the focus of attention in clinic. Methodshepatotoxicity was induced in rats using galn. This study investigated the protective effects of s. Oxidative stress and inflammation contributed to the propagation of acute liver injury ali. Carvacrol 2methyl5lmethyiethylphenol is a predominant monoterpenic phenol occuring in many essential oils of the family labiatae including, origanum, satureja, thymbra, thymus, and corydothymus species.
Hepg2 cell lines were used for in vitro study and dgalactosamine dgaln induced. In contrast, jnk2 plays a more critical role than jnk1 in inducing hepatocyte apoptosis in a model of con adgalactosamineinduced liver injury. Mice in each group n 6 were fed with a standard diet american institute of nutrition ain93g, a positive control diet containing 05 %. Nature and mechanisms of hepatocyte apoptosis induced by d. An international journal of pharmaceutical science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Hepatoprotective action of alcoholic extract of bacopa monniera bme was evaluated on dgalactosamine dgaln induced rat liver toxicity. Nacetyldgalactosamine is the denantiomer of nacetylgalactosamine. Acetylbergenin was synthesized from acetylation of bergenin, isolated from mallotus japonicus, to increase lipophilic and physiological activities. Ultrastructural features in galactosamineinduced hepatitis. Metabolomics, a branch of systems biology, is an important platform in the postgenomics era that focuses on small endogenous metabolites that are the final products of biological metabolite pathways nicholson and lindon 2008. Primary cultures of rat hepatocytes were exposed to 0.
Thus, the present study was to evaluate the effectiveness of piper cubeba fruits in the amelioration of ccl 4 induced liver injuries and oxidative damage in the rodent model. Protective effect of danhong injection on acute hepatic. The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galninduced liver damage in rats, compared with that of bergenin reported previously. Hepatoprotective activity of nigella sativa extract in d. To evaluate the hepatoprotective effect of ambrex, a polyherbal formulation against dgalactosamine dgln induced hepatotoxicity in swiss albino. Green tea extract supplement reduces dgalactosamine. Green tea extract supplement reduces dgalactosamineinduced acute liver injury by inhibition of apoptotic and proinflammatory signaling.
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